50.4 Folate (categories)

Folate range (<45.3 vs >=45.3)

50.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 130.8498286 1 <0.0001 ***
Folate_gp 1.5474194 1 0.2 ns
Study 0.0028985 1 1.0 ns
GA_samp 129.9037148 1 <0.0001 ***
Folate_gp:Study 0.1873616 1 0.7 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 31.8026411 1 <0.0001 ***
Folate_gp 0.5247425 1 0.5 ns
Study 15.3487117 1 <0.0001 ***
GA_samp 1637.3958932 1 <0.0001 ***
Folate_gp:Study 1.3445950 1 0.2 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 340.5884144 1 <0.0001 ***
Folate_gp 0.8284085 1 0.4 ns
Study 0.9187959 1 0.3 ns
GA_samp 809.0684784 1 <0.0001 ***
Folate_gp:Study 1.6021790 1 0.2 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 4.454 4.454 0.03 *
3 Study 1 Inf 10.576 10.576 0.001 **
4 GA_samp 1 Inf 0.113 0.113 0.7 ns
2 Folate_gp:Study 1 Inf 0.585 0.585 0.4 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 3.940 3.940 0.05 ns
3 Study 1 Inf 20.069 20.069 <0.0001 ***
4 GA_samp 1 Inf 0.199 0.199 0.7 ns
2 Folate_gp:Study 1 Inf 0.723 0.723 0.4 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 0.361 0.361 0.5 ns
3 Study 1 Inf 53.217 53.217 <0.0001 ***
4 GA_samp 1 Inf 0.198 0.198 0.7 ns
2 Folate_gp:Study 1 Inf 1.123 1.123 0.3 ns

50.4.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 12.92 (8.33, 20.02) <0.0001
Folate_gp≥45.3 0.93 (0.86, 1.02) 0.14
StudySTOP 1.01 (0.88, 1.16) 0.86
GA_samp 1.17 (1.14, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.92 (1.54, 2.41) <0.0001
Folate_gp≥45.3 0.99 (0.95, 1.04) 0.75
StudySTOP 1.14 (1.07, 1.23) 0.0002
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.08) <0.0001
Folate_gp≥45.3 0.99 (0.93, 1.06) 0.87
StudySTOP 1.03 (0.94, 1.13) 0.53
GA_samp 1.32 (1.29, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 16.05 (9.08, 28.36) <0.0001
Folate_gp≥45.3 0.88 (0.79, 0.99) 0.04
StudySTOP 0.71 (0.60, 0.86) 0.0003
GA_samp 0.99 (0.96, 1.03) 0.74
HOMA-IR
Var Effect P
(Intercept) 4.00 (2.21, 7.25) <0.0001
Folate_gp≥45.3 0.89 (0.78, 1.00) 0.06
StudySTOP 0.62 (0.51, 0.75) <0.0001
GA_samp 0.99 (0.95, 1.03) 0.66
Glucose (mmol/L)
Var Effect P
(Intercept) 5.53 (4.97, 6.16) <0.0001
Folate_gp≥45.3 1.01 (0.98, 1.04) 0.42
StudySTOP 0.87 (0.84, 0.90) <0.0001
GA_samp 1.00 (0.99, 1.01) 0.63

50.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 94.28120 7.881276 105.81912 79.88169 111.2764
STOP 95.46369 7.493420 82.64525 81.66396 111.5953
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.55766 2.703633 200.0909 84.38197 95.0508
STOP 102.42476 2.760721 126.5065 97.10473 108.0363
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.270358 0.0783020 457.2962 2.121580 2.429569
STOP 2.339495 0.0664836 239.7681 2.212127 2.474197
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 13.92731 0.8952145 Inf 12.278749 15.79722
STOP 9.94880 0.4661921 Inf 9.075784 10.90579
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.367075 0.2289763 Inf 2.946912 3.847143
STOP 2.078715 0.1024898 Inf 1.887239 2.289617
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.441858 0.0705402 Inf 5.305343 5.581886
STOP 4.733070 0.0429425 Inf 4.649648 4.817988

50.4.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥45.3 / <45.3 0.93 (0.86, 1.02) 0.1
hPL (μg/ml) ≥45.3 / <45.3 0.99 (0.95, 1.04) 0.8
GH2 (ng/ml) ≥45.3 / <45.3 0.99 (0.93, 1.06) 0.9
Insulin (uU/ml) ≥45.3 / <45.3 0.88 (0.79, 0.99) 0.04
HOMA-IR ≥45.3 / <45.3 0.89 (0.78, 1.00) 0.06
Glucose (mmol/L) ≥45.3 / <45.3 1.01 (0.98, 1.04) 0.4

50.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.4.6 Plots